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renilla luciferase reporter plasmid ptk-rl  (Promega)

 
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    Promega renilla luciferase reporter plasmid ptk-rl
    Renilla Luciferase Reporter Plasmid Ptk Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/renilla luciferase reporter plasmid ptk-rl/product/Promega
    Average 90 stars, based on 1 article reviews
    renilla luciferase reporter plasmid ptk-rl - by Bioz Stars, 2026-04
    90/100 stars

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    renilla luciferase reporter plasmid ptk-rl  (Promega)
    90
    Promega renilla luciferase reporter plasmid ptk-rl
    Renilla Luciferase Reporter Plasmid Ptk Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/renilla luciferase reporter plasmid ptk-rl/product/Promega
    Average 90 stars, based on 1 article reviews
    renilla luciferase reporter plasmid ptk-rl - by Bioz Stars, 2026-04
    90/100 stars
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    renilla luciferase reporter plasmid ptk‐rl  (Promega)
    90
    Promega renilla luciferase reporter plasmid ptk‐rl
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Renilla Luciferase Reporter Plasmid Ptk‐Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/renilla luciferase reporter plasmid ptk‐rl/product/Promega
    Average 90 stars, based on 1 article reviews
    renilla luciferase reporter plasmid ptk‐rl - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    ptk-rl plasmid  (Promega)
    90
    Promega ptk-rl plasmid
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Ptk Rl Plasmid, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptk-rl plasmid/product/Promega
    Average 90 stars, based on 1 article reviews
    ptk-rl plasmid - by Bioz Stars, 2026-04
    90/100 stars
    		  Buy from Supplier
    control plasmid ptk-rl  (Beyotime)
    90
    Beyotime control plasmid ptk-rl
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Control Plasmid Ptk Rl, supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/control plasmid ptk-rl/product/Beyotime
    Average 90 stars, based on 1 article reviews
    control plasmid ptk-rl - by Bioz Stars, 2026-04
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    ptk-rl plasmids  (Thermo Fisher)
    90
    Thermo Fisher ptk-rl plasmids
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Ptk Rl Plasmids, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptk-rl plasmids/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    ptk-rl plasmids - by Bioz Stars, 2026-04
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    renilla luciferase plasmid ptk-rl  (Promega)
    90
    Promega renilla luciferase plasmid ptk-rl
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Renilla Luciferase Plasmid Ptk Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/renilla luciferase plasmid ptk-rl/product/Promega
    Average 90 stars, based on 1 article reviews
    renilla luciferase plasmid ptk-rl - by Bioz Stars, 2026-04
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    plasmid ptk-rl  (Promega)
    90
    Promega plasmid ptk-rl
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Plasmid Ptk Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/plasmid ptk-rl/product/Promega
    Average 90 stars, based on 1 article reviews
    plasmid ptk-rl - by Bioz Stars, 2026-04
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    transfection control renilla luciferase plasmid ptk-rl  (Promega)
    90
    Promega transfection control renilla luciferase plasmid ptk-rl
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Transfection Control Renilla Luciferase Plasmid Ptk Rl, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/transfection control renilla luciferase plasmid ptk-rl/product/Promega
    Average 90 stars, based on 1 article reviews
    transfection control renilla luciferase plasmid ptk-rl - by Bioz Stars, 2026-04
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    ptk rl plasmid 21–170  (Millipore)
    90
    Millipore ptk rl plasmid 21–170
    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative <t>luciferase</t> (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by <t>Renilla</t> luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.
    Ptk Rl Plasmid 21–170, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ptk rl plasmid 21–170/product/Millipore
    Average 90 stars, based on 1 article reviews
    ptk rl plasmid 21–170 - by Bioz Stars, 2026-04
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    Image Search Results


    Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative luciferase (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by Renilla luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.

    Journal: Cancer Science

    Article Title: Application of prime editing system to introduce TP53 R248Q hotspot mutation in acute lymphoblastic leukemia cell line

    doi: 10.1111/cas.16162

    Figure Lengend Snippet: Transactivation activity and downstream target gene expression of p53 protein. (A) Reporter assay of p53 protein. Top panel: sequences of WT and mutated p53 responsive elements in response element (RE; upper) and disrupted response element (dRE; lower) reporter plasmids are indicated. Bottom panel: relative luciferase (Luc) activities in acute lymphoblastic leukemia parental cells (left) and the subline (right) cultured in the presence (+) or absence (−) of 5 μM nutlin‐3a for 6 h. Luminescence produced by Renilla luciferase was used as an internal standard. Error bars indicate SD in triplicate analyses. BAS, basic; CTL, pGL3‐control; dRE, pGL3‐dRE; RE, pGL3‐RE. (B) Changes in the gene expression levels of CDKN2A (upper panel) and PUMA (lower panel). Real‐time RT‐PCR analysis was carried out in parental cells (left) and the subline (right) cultured in the presence or absence of nutlin‐3a at 5 μM for the indicated periods using the ACTB gene expression level as an internal control. Error bars indicate SD in triplicate analyses.

    Article Snippet: Renilla luciferase reporter plasmid pTK‐RL (E2231; Promega) was cotransfected as the internal standard.

    Techniques: Activity Assay, Targeted Gene Expression, Reporter Assay, Luciferase, Cell Culture, Produced, Expressing, Quantitative RT-PCR